TaqMan™ Universal Master Mix II, with UNG - 4440038
Marca | Applied Biosystems™ |
Línea de producto | TaqMan™ |
Referencia | 4440038 |
Tamaño / cantidad | 1 x 5 ml |
Condiciones de embalaje | Hielo húmedo |
Este producto también se encuentra con las siguientes referencias:
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components
Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.
TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays
For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.
Alternative product:
Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.
For Use With (Equipment): 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye: ROX (Pre-mixed)
Primer Probe Compatibility: TaqMan®
Product Line: TaqMan™
Purity or Quality Grade: UP (Ultra Pure)
Quantity: 1 x 5 mL
Sample Type: DNA (Genomic), cDNA
Shipping Condition: Wet Ice
Detection Method: Primer-Probe Detection
PCR Method: qPCR
Polymerase: AmpliTaq Gold DNA Polymerase
Reaction Speed: Standard
Contents & storage
Store at 4°C
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Sólo para uso investigativo. No utilizar en procedimientos diagnósticos